DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using GeneChip™ Hybridization, Wash, and Stain Kit to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail
Get tips on using Genomic DNA isolation to perform DNA isolation / purification Cells - Immortalized cell lines Leiomyoma & myometrial cells
Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Tissue - bone
Get tips on using Genomic DNA MiniSpin: Bacteria to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas
Get tips on using Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Salmonella enterica
Get tips on using Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus
Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment